Human Teratocarcinoma Derived Growth Factor 1 ELISA Kit

Designed for the in vitro quantitative determination of human Cripto1/CRIPTO (Teratocarcinoma Derived Growth Factor 1), this sandwich ELISA from Antibodies.com uses a pre-coated 96-well microplate (12 x 8 well strips) with a colourmetric readout at 450 nm. Validated for serum, plasma, tissue homogenates, cell lysates and other biological fluids, the assay spans a standard range of 0.156–10 ng/ml with a sensitivity of <0.053 ng/ml and a total run time of 4 hours 30 minutes.

Kit components — lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers — are stored at +4°C or −20°C as indicated. Spike-and-recovery has been characterised in serum, EDTA plasma and heparin plasma, with average recoveries of 91%, 90% and 84% respectively, and dilution linearity demonstrated from 1:2 to 1:16 across all three sample types.