Human Tau-Tubulin Kinase 2 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human TTBK2 (Tau-Tubulin Kinase 2) in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate and a biotinylated detection antibody followed by HRP-Avidin and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The reported assay range is 0.312-20 ng/ml with a sensitivity of 0.124 ng/ml, and the total assay time is 4h 30m. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Sample recovery has been characterised in serum, EDTA plasma and heparin plasma at dilutions from 1:2 to 1:16. Average recoveries in neat samples ranged from 84% in heparin plasma to 91% in serum, with EDTA plasma averaging 90%. Across serial dilutions, recoveries remained within the ranges reported for each matrix.