Human TANK Binding Kinase 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human TANK Binding Kinase 1 (NAK/TBK1) in tissue homogenates, cell lysates, and other biological fluids, with reactivity confirmed for human samples. The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-NAK/TBK1 detection antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The stated assay range is 0.312-20 ng/ml and the sensitivity is <0.112 ng/ml, with a total assay time of 4h 30m. Reported recovery values (n=5) are 80-102% (average 91%) for serum, 81-100% (average 90%) for EDTA plasma, and 80-89% (average 84%) for heparin plasma. Dilution linearity has been characterised at 1:2, 1:4, 1:8, and 1:16 dilutions across serum, EDTA plasma, and heparin plasma samples. Kit components are stored at +4°C or -20°C as indicated, and the kit should not be used past its expiration date.