Human Syntaxin 1A, Brain ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the quantitative determination of human Syntaxin 1A in tissue homogenates, cell lysates, and other biological fluids. It uses a pre-coated 96-well microplate configured as 12 x 8 well strips and employs a biotinylated detection antibody with an HRP-Avidin conjugate, with signal developed using TMB substrate and read at 450 nm. The reported assay range is 0.156–10 ng/ml with a sensitivity of 0.056 ng/ml, and the complete protocol takes 4h 30m.

Recovery values from spiked samples are reported as 83–100% (average 91%) for serum, 90–103% (average 96%) for EDTA plasma, and 89–102% (average 95%) for heparin plasma. The product is intended for research use only and is not for diagnostic or therapeutic applications.