Human Synergin Gamma ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com enables the in vitro quantitative determination of human SYNRG (Synergin Gamma) in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate with an immobilized capture antibody specific for SYNRG, followed by a biotinylated anti-SYNRG detection antibody, HRP-avidin conjugate, and TMB substrate. The resulting colour change is proportional to SYNRG concentration and is read at 450 nm on a colorimetric microplate reader.

Reported performance characteristics include an assay range of 0.312-20 ng/ml and a sensitivity of <0.109 ng/ml, with a total assay time of 4h 30m.

Matrix validation data are provided for serum (average recovery 91%, range 80-102%, n=5), EDTA plasma (90%, 81-100%, n=5), and heparin plasma (84%, 80-89%, n=5), alongside dilution linearity assessed at 1:2, 1:4, 1:8, and 1:16 for each matrix. The kit is supplied with a pre-coated microplate, lyophilized standard, detection reagents, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, intended for storage at +4°C or -20°C as instructed.