Human Suppressor Of Zeste 12 Homolog ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com, designed for the in vitro quantitative determination of human SUZ12 (Suppressor of Zeste 12 Homolog) in tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, using an antibody specific for SUZ12 pre-coated onto a microtitre plate. Standards and test samples are added and SUZ12 present in each sample is captured by the immobilised antibody. Following incubation and washing, a Biotinylated Anti-SUZ12 Antibody binds the captured SUZ12, then HRP-Avidin conjugate is added. After further incubation and washing, a TMB substrate solution visualises the HRP enzymatic reaction, producing a blue product that turns yellow upon addition of acidic stop solution. The density of yellow is proportional to the SUZ12 concentration, calculated from O.D. absorbance at 450 nm using a standard curve.

The Human Suppressor Of Zeste 12 Homolog ELISA Kit reports a sensitivity of <0.113 ng/ml and an assay range of 0.312–20 ng/ml, with a total assay time of 4h 30m. Detection is colourimetric on a pre-coated microplate. Average recovery values from spiked samples were 91% in serum (n=5, range 83–100%), 96% in EDTA plasma (range 90–103%), and 95% in Heparin plasma (range 89–102%). Dilution linearity across 1:2 to 1:16 was demonstrated for serum (82–110%), EDTA plasma (73–109%), and Heparin plasma (77–110%). Kit components should be stored at +4°C or −20°C as instructed and the kit should not be used past its expiration date.