Human Superoxide Dismutase 2, Mitochondrial ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is intended for the in vitro quantitative determination of human SOD2/MnSOD in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, with an antibody specific for SOD2/MnSOD pre-coated onto a 96-well microtiter plate configured as 12 x 8 well strips. Standards and test samples are added to the wells and SOD2/MnSOD is bound by the immobilized antibody. After washing, a Biotinylated Anti-SOD2/MnSOD Antibody binds the captured analyte, followed by HRP-Avidin conjugate. TMB substrate is added to visualize the HRP enzymatic reaction as a blue-coloured product that turns yellow upon addition of acidic stop solution, with the density of yellow proportional to the amount of SOD2/MnSOD captured. The analyte concentration is calculated by reading O.D. absorbance at 450 nm against the standard curve.

The product range is 0.781–50 ng/ml with a sensitivity of <0.29 ng/ml. Assay time is 4h 30m and detection is colourimetric, performed on a pre-coated microplate platform. Supplied components include a pre-coated 96-well microplate, lyophilized standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers. Components are stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.

Reported recovery values: Serum (n=5) range 80%–102%, average 91%; EDTA Plasma (n=5) range 81%–100%, average 90%; Heparin Plasma (n=5) range 80%–89%, average 84%. Dilution linearity across 1:2, 1:4, 1:8 and 1:16 dilutions is reported as: Serum (n=5) 87–91%, 87–107%, 74–101%, 92–97%; EDTA Plasma (n=5) 90–105%, 84–101%, 90–101%, 79–108%; Heparin Plasma (n=5) 84–95%, 92–105%, 82–105%, 89–91%.