Human Sulfatase 1 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human Sulfatase 1/SULF1 in serum, plasma, tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate (12 x 8 well strips), with detection performed colorimetrically by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Reported performance figures include an assay range of 1.56-100 ng/ml and a sensitivity of <0.62 ng/ml. The total assay time is 4h 30m. Kit components should be stored at +4°C or -20°C as instructed, and the kit is intended for use with a Colorimetric Microplate Reader before the stated expiration date. The reactive target is Sulfatase 1/SULF1, and the kit is suitable for serum, plasma and tissue homogenates.

Spike-and-recovery data are provided for serum (n=5, range 80%-102%, average 91%), EDTA plasma (n=5, range 81%-100%, average 90%) and heparin plasma (n=5, range 80%-89%, average 84%), alongside linearity data across 1:2, 1:4, 1:8 and 1:16 dilutions for each sample type.