Human Steroid 5 Alpha Reductase 2 ELISA Kit

Manufactured for Antibodies.com, this sandwich enzyme-linked immunosorbent assay is designed for the in vitro quantitative determination of human SRD5A2 in serum, plasma, tissue homogenates or other biological fluids. The kit uses a pre-coated microplate with biotinylated anti-SRD5A2 detection antibody, HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

Reported sensitivity is 0.126 ng/ml with an assay range of 0.312–20 ng/ml, and total assay time is 4h 30m. Kit components are stored at +4°C or −20°C as instructed, and reagents should not be used past the expiration date.

Spike-and-recovery performance is documented for serum (average 91%, range 80–102%), EDTA plasma (average 91%, range 81–101%) and heparin plasma (average 84%, range 80–89%), with dilution linearity reported across 1:2 to 1:16 dilutions for all three matrices.