Human Stem Cell Factor Receptor ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) supplied by Antibodies.com for the in vitro quantitative determination of human c-Kit (Stem Cell Factor Receptor) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The procedure uses a pre-coated 96-well microplate and a biotinylated anti-c-Kit detection antibody, followed by an HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm. Total assay time is 4 hours 30 minutes, the stated product range is 0.156-10 ng/ml, and the sensitivity is 0.052 ng/ml. Kit components should be stored at +4°C or -20°C as instructed.

Manufacturer validation data: spike-and-recovery in serum (n=5) was 80%-102% with an average of 91%; in EDTA plasma (n=5) was 81%-100% with an average of 90%; and in heparin plasma (n=5) was 80%-89% with an average of 84%. Dilution linearity at 1:2, 1:4, 1:8 and 1:16 was 87-91%, 87-107%, 74-101% and 92-97% for serum (n=5); 90-105%, 84-101%, 90-101% and 79-108% for EDTA plasma (n=5); and 84-95%, 92-105%, 82-105% and 89-91% for heparin plasma (n=5).