Human Spondin 1 ELISA Kit

A sandwich ELISA kit from Antibodies.com designed for the in vitro quantitative determination of human F-Spondin in serum, plasma, or other biological fluids. The assay employs a pre-coated microplate with a biotinylated anti-F-Spondin detection antibody and HRP-avidin conjugate, producing a colorimetric signal via TMB substrate that is read at 450 nm. Sensitivity is <0.212 ng/ml and the assay range is 0.625–40 ng/ml. Total assay time is 4h 30m.

Kit components include a pre-coated microplate (12 x 8 well strips), lyophilized standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, with storage at +4°C or -20°C as instructed. Recovery data are reported for serum (80–102%, average 91%), EDTA plasma (81–100%, average 90%), and heparin plasma (80–89%, average 84%), with linearity assessed across 1:2, 1:4, 1:8, and 1:16 dilutions. Reagents should not be used past the expiration date.