Human Spectrin Beta, Non Erythrocytic 4 ELISA Kit

A sandwich ELISA kit from Antibodies.com for the in vitro quantitative determination of human Spectrin Beta, Non Erythrocytic 4 in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate, a biotinylated detection antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The stated product range is 0.156–10 ng/ml with a sensitivity of <0.063 ng/ml, and the total assay time is 4 hours 30 minutes. Components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers. Store kit components at +4°C or -20°C as instructed and do not use past the expiration date.

Spike-and-recovery across n=5 averaged 91% in serum (range 80–102%), 91% in EDTA plasma (81–101%), and 84% in heparin plasma (80–89%). Dilution linearity from 1:2 to 1:16 spanned 87–107% (serum), 79–108% (EDTA plasma), and 82–105% (heparin plasma). Detection is colourmetric on a pre-coated microplate platform.