Human Sirtuin 6 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay supplied by Antibodies.com for the in vitro quantitative determination of human Sirtuin 6 (SIRT6). The kit is reactive with human samples and is suitable for serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. The assay uses a pre-coated 96-well microplate formatted as 12 x 8 well strips and a colorimetric TMB substrate, with absorbance read at 450 nm in a microplate reader.

The reported product range is 78.1–5000 pg/ml with a sensitivity of <30.6 pg/ml, and the total assay time is 4 hours 30 minutes. Reported spike-and-recovery values are 80–102% for serum (average 91%, n=5), 81–100% for EDTA plasma (average 90%, n=5), and 80–89% for heparin plasma (average 84%, n=5). Kit components should be stored at +4°C or -20°C as indicated, and the kit must not be used past its expiration date.