Human Signal Transducer And Activator Of Transcription 6 ELISA Kit

This sandwich ELISA from Antibodies.com enables in vitro quantitative determination of human Signal Transducer and Activator of Transcription 6 (STAT6) in tissue homogenates or other biological fluids. The kit is supplied as a pre-coated 96-well microplate arranged as 12 x 8 well strips and uses a colourimetric readout captured at 450 nm on a standard microplate reader, with a total assay time of 4 hours 30 minutes.

An antibody specific for STAT6 is immobilised on the plate. Standards and samples are added to the wells, followed by a biotinylated anti-STAT6 antibody and then an HRP-avidin conjugate. TMB substrate is used to visualise the enzymatic reaction, producing a blue product that becomes yellow upon addition of acidic stop solution; the absorbance intensity is proportional to STAT6 concentration and is quantified against the supplied standard curve. Reported sensitivity is less than 0.128 ng/ml and the assay range is 0.312-20 ng/ml. Kit components are stored at +4°C or -20°C as instructed.

Validation studies report average recoveries of 91% in serum (range 80-102%, n=5), 90% in EDTA plasma (range 81-100%, n=5), and 84% in heparin plasma (range 80-89%, n=5). Linearity was confirmed across dilutions of 1:2 to 1:16 in all three sample types.