Human Sialic Acid Binding Ig Like Lectin 8 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Siglec 8 (Sialic Acid Binding Ig Like Lectin 8) in cell lysates, read on a colorimetric microplate reader. The assay has a working range of 0.156–10 ng/ml and a sensitivity of 0.058 ng/ml, with absorbance read at 450 nm against a standard curve. Total assay time is 4h 30m on a pre-coated 96-well microplate platform.

Reported recovery for spiked samples covers 80–102% for serum, 81–101% for EDTA plasma and 80–89% for heparin plasma, with dilution linearity data supplied across 1:2 to 1:16 dilutions. The procedure uses a biotinylated anti-Siglec 8 detection antibody followed by an HRP-avidin conjugate and TMB substrate; the catalysed reaction is stopped with an acidic solution to give a yellow product whose absorbance is proportional to Siglec 8 concentration in each well.