Human Sialic Acid Binding Ig Like Lectin 10 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Siglec 10 (Sialic Acid Binding Ig Like Lectin 10) in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and the sandwich enzyme immunoassay technique, with detection via an HRP-Avidin conjugate and TMB substrate. Absorbance is read at 450 nm on a colorimetric microplate reader.

Reported performance includes an assay range of 0.156–10 ng/ml and a sensitivity of <0.062 ng/ml, with a total assay time of 4h 30m. Sample recovery averages 91% for serum (range 83%–100%), 94% for EDTA plasma (range 90%–98%), and 95% for heparin plasma (range 89%–102%), each evaluated at n=5. Linearity has been demonstrated at dilutions of 1:2, 1:4, 1:8, and 1:16 across serum, EDTA plasma, and heparin plasma. Kit components are stored at +4°C or –20°C as instructed, and the kit should not be used past its expiration date.