Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com for the in vitro quantitative determination of human SPLUNC3 (Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3) in tissue homogenates and other biological fluids. The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips and is intended for use with human samples.

The protocol uses a Biotinylated Anti-SPLUNC3 Antibody and an HRP-Avidin conjugate, with TMB substrate yielding a coloured product read at 450nm on a colorimetric microplate reader. The source material reports an assay range of 0.156-10 ng/ml, a sensitivity of <0.066 ng/ml, and a total assay time of 4h 30m.

Recovery data (n=5) from the source material include 80%-102% with an average of 91% for serum, 81%-101% with an average of 91% for EDTA plasma, and 80%-89% with an average of 84% for heparin plasma. Linearity has been evaluated across dilutions of 1:2, 1:4, 1:8 and 1:16 for each sample type. Kit components are stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date.