Human Sex Determining Region Y Box Protein 18 ELISA Kit

A sandwich ELISA from Antibodies.com intended for the in vitro quantitative determination of human SOX18 in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) and is read on a colorimetric microplate reader.

Quoted performance specifications include a product range of 0.156-10 ng/ml and a sensitivity of <0.041 ng/ml, with an assay time of 4h 30m. Reported average recovery values across the stated sample types are 91% for serum, 96% for EDTA plasma and 95% for heparin plasma.

Storage directions instruct users to keep kit components at +4°C or -20°C as instructed and not to use past the expiration date. The procedure involves addition of standards and samples to antibody-coated wells, incubation with Biotinylated Anti-SOX18 Antibody, addition of HRP-Avidin conjugate, and visualisation via TMB substrate with absorbance read at 450nm.