Human Serine Peptidase Inhibitor Kunitz Type 2 ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human Serine Peptidase Inhibitor Kunitz Type 2 (HGFA Inhibitor 2) in serum, plasma and other biological fluids. The procedure uses a pre-coated 96-well microplate (12 x 8 well strips) and the sandwich enzyme immunoassay technique, with a biotinylated anti-HGFA Inhibitor 2 detection antibody and an HRP-avidin conjugate. Signal is generated using a TMB substrate, with the reaction read as absorbance at 450 nm on a colorimetric microplate reader.

The kit is reactive with human samples. The manufacturer reports recovery data for serum (average 91%, range 80-102%, n=5), EDTA plasma (average 91%, range 81-101%, n=5) and heparin plasma (average 84%, range 80-89%, n=5), and provides dilution linearity information for 1:2, 1:4, 1:8 and 1:16 sample dilutions. Kit components include pre-coated strips, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed.

Stated performance characteristics include an assay range of 0.312-20 ng/ml, a sensitivity of <0.103 ng/ml and a total assay time of 4h 30m. The assay type is sandwich (quantitative) with colorimetric readout.