Human Serine Peptidase Inhibitor Kazal Type 1 ELISA Kit

Antibodies.com offers a sandwich ELISA kit for the in vitro quantitative determination of human Serine Peptidase Inhibitor Kazal Type 1 (SPINK1/P12) in serum, plasma or other biological fluids. The assay has been validated for reactivity with human samples and uses the sandwich enzyme immunoassay technique on a pre-coated microplate platform.

The standard curve covers a detection range of 1.56–100 ng/ml with a sensitivity of <0.62 ng/ml. Detection is colorimetric, read at 450 nm in a microplate reader, and total assay time is 4 h 30 m. Kit components are intended for storage at +4 °C or -20 °C as instructed, and the reagents should not be used past the stated expiration date.

Reported recovery is approximately 91% in serum (range 80–102%), 91% in EDTA plasma (range 81–101%) and 84% in heparin plasma (range 80–89%). Linearity across serial two-fold dilutions has been demonstrated in serum, EDTA plasma and heparin plasma.