Human Secreted And Transmembrane Protein 1 ELISA Kit

From Antibodies.com, this human SECTM1 (Secreted And Transmembrane Protein 1) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human SECTM1 in tissue homogenates, urine and other biological fluids. The assay uses a 96-well microtiter plate pre-coated with an antibody specific for SECTM1, with detection via a biotinylated anti-SECTM1 antibody, an HRP-avidin conjugate and TMB substrate. The enzymatic reaction produces a blue-coloured product that changes to yellow upon addition of acidic stop solution, and the concentration of SECTM1 is calculated from the O.D. absorbance read at 450nm in a microplate reader against a standard curve. Reactivity is Human.

Stated sensitivity is 0.27 ng/ml and the assay measuring range is 0.781-50 ng/ml. Total assay time is approximately 4 hours 30 minutes. Reported average recovery values are 91% for serum (range 80-102%, n=5), 91% for EDTA plasma (range 81-101%, n=5) and 84% for heparin plasma (range 80-89%, n=5), with linearity assessed across sample dilutions of 1:2, 1:4, 1:8 and 1:16 in the same matrices. Kit components include a pre-coated 96 well microplate (12 x 8 well strips), lyophilised standard, Detection Solution A, Detection Solution B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, to be stored at +4°C or -20°C as instructed. The product is for research use only.