Human S-Phase Kinase Associated Protein 1 ELISA Kit

Antibodies.com supplies a sandwich ELISA kit for the in vitro quantitative determination of human SKP1 in tissue homogenates, cell lysates and other biological fluids. The assay employs the sandwich enzyme immunoassay technique using a pre-coated microplate, a Biotinylated Anti-SKP1 Antibody and an HRP-Avidin conjugate, with TMB substrate producing a colorimetric signal read at 450 nm in a microplate reader.

The kit's reported assay range is 0.156-10 ng/ml with a sensitivity of <0.054 ng/ml, and the total assay time is 4 hours 30 minutes. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Validation testing reports average recovery values of 91% for serum (range 80-102%, n=5), 90% for EDTA plasma (range 81-100%, n=5) and 84% for heparin plasma (range 80-89%, n=5). Dilution linearity tested at 1:2, 1:4, 1:8 and 1:16 gave overall recovery ranges of 74-107% for serum (n=5), 79-108% for EDTA plasma (n=5) and 82-105% for heparin plasma (n=5). The kit is suitable for cell lysates and tissue homogenates.