Human Ribonuclease A7 ELISA Kit

Manufactured by Antibodies.com, this human ribonuclease assay is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human RNase 7 in serum, plasma, tissue homogenates or other biological fluids. The kit employs the sandwich enzyme immunoassay technique, using an antibody specific for RNase 7 pre-coated onto a 96-well microplate. Standards and samples are added, followed by incubation with a Biotinylated Anti-RNase 7 Antibody and then an HRP-Avidin conjugate, with TMB substrate used to visualize the reaction. The catalysis produces a blue-coloured product that changes to yellow upon addition of an acidic stop solution, and the concentration of RNase 7 is calculated from the O.D. absorbance read at 450 nm in a microplate reader, by reference to a standard curve.

The assay is intended for use on a Colorimetric Microplate Reader. The stated sensitivity is 0.61 ng/ml and the assay range is 1.56-100 ng/ml. The platform is a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a total assay time of 4h 30m. Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.

Recovery performance has been evaluated across sample types. In serum (n=5) the average recovery was 91% (range 80-102%); in EDTA plasma (n=5) it was 91% (range 81-101%); and in heparin plasma (n=5) it was 84% (range 80-89%). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 dilutions was reported as 87-91%, 87-107%, 74-101% and 92-97% for serum (n=5); 90-105%, 84-101%, 90-101% and 79-108% for EDTA plasma (n=5); and 84-95%, 92-105%, 82-105% and 89-91% for heparin plasma (n=5).