Human Replication Initiator 1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com for the quantitative determination of human Replication Initiator 1 (REPIN1) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated microplate with an immobilized antibody specific for REPIN1, a biotinylated anti-REPIN1 detection antibody, and an HRP-Avidin conjugate. Signal is visualised with TMB substrate, producing a yellow product whose density is proportional to REPIN1 concentration; absorbance is read at 450 nm on a colorimetric microplate reader. Reported sensitivity is 0.129 ng/ml and the assay range is 0.312–20 ng/ml. The total assay time is 4 hours 30 minutes. Kit components should be stored at +4°C or −20°C as instructed, and the product is intended for research use only.