Human Receptor Activity Modifying Protein 2 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables in vitro quantitative determination of human Receptor Activity Modifying Protein 2 (RAMP2), with reactivity validated for human samples and suitability for tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate, with standards and samples incubated alongside a Biotinylated Anti-RAMP2 Antibody, an HRP-Avidin conjugate, and TMB substrate. The colour change, read at 450 nm on a colorimetric microplate reader, is proportional to RAMP2 concentration and is quantified against a standard curve.

Reported performance characteristics include a sensitivity of <0.059 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4h 30m. Validation data for serum, EDTA plasma, and heparin plasma show average recovery values of 91%, 90%, and 84% respectively. Kit components should be stored at +4°C or -20°C as instructed, and the product must not be used past its expiration date.