Human RCC1 And BTB Domain Containing Protein 2 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human RCC1 and BTB Domain Containing Protein 2 in tissue homogenates or other biological fluids. The assay employs a sandwich immunoassay format using a pre-coated microplate, where targets are bound by an immobilized antibody and subsequently detected using a Biotinylated Antibody, HRP-Avidin conjugate, and TMB substrate. The resulting colour change is read at 450nm.

The assay has a total run time of 4h 30m and operates on a colourmetric detection platform. It demonstrates a sensitivity of <0.124 ng/ml and a measuring range of 0.312-20 ng/ml. Reported average recovery rates are 91% for Serum (range 80% - 102%), 91% for EDTA Plasma (range 81% - 101%), and 84% for Heparin Plasma (range 80% - 89%). Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.