Human RalA Binding Protein 1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human RALBP1 (RalA Binding Protein 1) in tissue homogenates, cell lysates and other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for RALBP1, with bound analyte detected using a Biotinylated Anti-RALBP1 Antibody and HRP-Avidin conjugate, visualised with TMB substrate that produces a yellow product upon addition of acidic stop solution.

Quantitative readout is performed on a colorimetric microplate reader at 450 nm, with concentration calculated against the standard curve. Reported assay performance characteristics include a measuring range of 0.156-10 ng/ml, a sensitivity of <0.058 ng/ml, and an assay time of 4h 30m. The platform is a pre-coated microplate arranged as 12 x 8 well strips, and the kit is suitable for cell lysates and tissue homogenates.

Spike-and-recovery data from the manufacturer covers serum, EDTA plasma and heparin plasma samples, with reported recovery ranges across dilution factors (1:2 to 1:16) and average recoveries of 91% (serum), 91% (EDTA plasma) and 84% (heparin plasma) at n=5. Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage indicated at +4°C or -20°C depending on the item.