Human Rac-GTPase Activating Protein 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human RACGAP1 (also referred to as MGCRACGAP), the protein formally designated Rac GTPase Activating Protein 1. The assay accepts serum, plasma, tissue homogenates, cell lysates and other biological fluids, with absorbance read on a colorimetric microplate reader at 450 nm. The manufacturer-quoted product range is 78.1–5000 pg/ml with a sensitivity of <25.8 pg/ml, and the full protocol runs for 4h 30m.

Manufacturer datasheet recovery data (n=5 per matrix) report averages of 91% in serum (range 80–102%), 91% in EDTA plasma (range 81–101%) and 84% in heparin plasma (range 80–89%), with corresponding dilution-linearity values supplied for 1:2, 1:4, 1:8 and 1:16 dilutions across the same three matrices. Kit components include a pre-coated microplate, lyophilised standard, biotinylated anti-RACGAP1 detection antibody, HRP-avidin conjugate, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, to be stored at +4°C or -20°C as instructed.