Human RAB5A, Member RAS Oncogene Family ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human RAB5A in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate with 12 x 8 well strips, an antibody specific for RAB5A, a Biotinylated Anti-RAB5A detection antibody and an HRP-Avidin conjugate, with visualisation via TMB substrate read at 450 nm. The stated sensitivity is <0.122 ng/ml and the assay range is 0.312–20 ng/ml, with a total assay time of 4h 30m. Reported average recovery values are 91% for serum (range 83–100%), 94% for EDTA plasma (range 90–99%) and 96% for heparin plasma (range 91–101%). Dilution linearity data are provided for serum, EDTA plasma and heparin plasma at 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components are stored at +4°C or -20°C as instructed, and the product is for research use only.