Human Protocadherin 15 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Protocadherin 15 (PCDH15) in tissue homogenates, cell lysates, and other biological fluids. The kit uses a pre-coated microplate with a biotinylated anti-PCDH15 detection antibody and HRP-avidin conjugate, and a TMB substrate that produces a colour change read at 450 nm in a colorimetric microplate reader. The standard curve covers an assay range of 0.312–20 ng/ml with a sensitivity of <0.105 ng/ml, and the total assay time is 4 hours 30 minutes.

Validation data indicate reliable recovery across multiple sample matrices, including serum (average 91%, n=5), EDTA plasma (94%, n=5), and heparin plasma (95%, n=5). Kit components are stored at +4°C or −20°C as instructed, and the kit should not be used past the stated expiration date.