Human Protein Tyrosine Phosphatase Like Protein A ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human Protein Tyrosine Phosphatase Like Protein A (PTPLA) in tissue homogenates, cell lysates, and other biological fluids, and is intended for use with human samples. The assay uses an antibody specific for PTPLA pre-coated onto a microplate, paired with a biotinylated detection antibody and HRP-avidin conjugate, with signal developed using TMB substrate and read at 450 nm in a colorimetric microplate reader.

According to the manufacturer's specifications, the assay has a working range of 0.781–50 ng/ml and a sensitivity of <0.25 ng/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery testing in human samples gave average recoveries of 91% in serum (range 80–102%), 90% in EDTA plasma (range 81–100%), and 84% in heparin plasma (range 80–89%). Dilution linearity was assessed across 1:2, 1:4, 1:8, and 1:16 dilutions, with overall reported ranges of 74–107% for serum, 79–108% for EDTA plasma, and 82–105% for heparin plasma.