Human Protein Phosphatase 1, Regulatory Subunit 1B ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA is designed for the quantitative in vitro determination of human Protein Phosphatase 1, Regulatory Subunit 1B (DARPP32) in tissue homogenates, cell lysates, or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) format with a biotinylated anti-DARPP32 detection antibody and HRP-Avidin/TMB substrate visualisation read at 450 nm. Total assay time is 4 hours 30 minutes, and the kit is intended for use with a colorimetric microplate reader.

Quoted performance figures include a sensitivity of <0.072 ng/ml and an assay range of 0.156–10 ng/ml. Kit components comprise the pre-coated microplate, lyophilised standard (2 vials), Detection Solution A (120 µl), Detection Solution B (120 µl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and 5 adhesive plate sealers. Storage temperatures indicated in the product literature are +4°C or –20°C as instructed per component.

Reported average sample recovery values (n=5) are 91% for serum (range 83–100%), 94% for EDTA plasma (range 90–98%), and 95% for heparin plasma (range 89–102%). Dilution linearity data is supplied across 1:2, 1:4, 1:8, and 1:16 dilutions for serum, EDTA plasma, and heparin plasma samples.