Human Protein Kinase C Beta 1 ELISA Kit

A sandwich-format ELISA from Antibodies.com is intended for the in vitro quantitative determination of human Protein Kinase C Beta 1 in serum, plasma, tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate configured as 12 x 8 well strips and relies on a biotinylated detection antibody with HRP-avidin and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The standard assay time is 4h 30m, with a stated product range of 0.156–10 ng/ml and a sensitivity of <0.06 ng/ml. Spiking recovery across the listed sample types averages 91% for serum, 89% for EDTA plasma, and 90% for heparin plasma, and linearity is demonstrated across 1:2, 1:4, 1:8, and 1:16 dilutions. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.