Human Protein Kinase B Gamma ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables in vitro quantitative determination of human AKT3 — also referred to as Protein Kinase B gamma, PKB gamma, PKBG, Protein kinase Akt-3, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, and STK-2 — in tissue homogenates, cell lysates, cell culture supernates, or other biological fluids.

The assay is run on a pre-coated 96-well microplate (12 x 8 well strips) with colorimetric detection read at 450 nm, and has a total assay time of 4h 30m. The stated measuring range is 1.56-100 ng/ml with a sensitivity of <0.56 ng/ml. Components are stored at +4°C or -20°C as instructed and should not be used past the expiration date.

Reported recovery data include Serum (n=5) overall range 80-102% (average 91%), EDTA Plasma (n=5) range 81-100% (average 90%), and Heparin Plasma (n=5) range 80-89% (average 84%). At dilutions of 1:2, 1:4, 1:8, and 1:16, Serum recovery ranges were 87-91%, 87-107%, 74-101%, and 92-97%; EDTA Plasma ranges were 90-105%, 84-101%, 90-101%, and 79-108%; Heparin Plasma ranges were 84-95%, 92-105%, 82-105%, and 89-91%.