Human Protein Disulfide Isomerase A2 ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human PDIA2/PDIP (Protein Disulfide Isomerase A2) in tissue homogenates or other biological fluids. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a colorimetric readout measured at 450 nm on a microplate reader.

Reported sensitivity is <0.113 ng/ml with an assay range of 0.312-20 ng/ml, and the total assay time is 4h 30m. Kit components are stored at +4°C or -20°C as instructed and must not be used past the expiration date. Contents include a pre-coated 96 well microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers.

Validation data are provided for serum, EDTA plasma, and heparin plasma. Average recovery values are 91% for serum (range 80-102%, n=5), 90% for EDTA plasma (range 81-100%, n=5), and 84% for heparin plasma (range 80-89%, n=5). Linearity at dilutions 1:2, 1:4, 1:8, and 1:16 is reported as: serum 87-91%, 87-107%, 74-101%, 92-97%; EDTA plasma 90-105%, 84-101%, 90-101%, 79-108%; heparin plasma 84-95%, 92-105%, 82-105%, 89-91%.