Human Protein C Receptor, Endothelial ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit from Antibodies.com is designed for the in vitro quantitative determination of human EPCR/CD201 (Protein C Receptor, Endothelial) in serum, plasma, or other biological fluids [C0, S1]. The kit falls within the Assays and Kits category and is reactive with human samples [C0].

Assay principle: a microplate is pre-coated with an antibody specific for EPCR/CD201. Standards and samples are added to the wells, allowing the target to be captured by the immobilized antibody. After washing, a Biotinylated Anti-EPCR/CD201 Antibody is added, followed by an HRP-Avidin conjugate. TMB substrate is then used to visualise the HRP enzymatic reaction, producing a blue product that turns yellow upon addition of acidic stop solution. Absorbance is read at 450 nm in a colorimetric microplate reader, and the concentration of EPCR/CD201 is calculated against the standard curve [S1]. The reported sensitivity is <0.275 ng/ml and the assay range is 0.625-40 ng/ml [S1]. Total assay time is approximately 4 hours 30 minutes [S1].

Reported recovery averages are 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-100%), and 84% for heparin plasma (range 80%-89%) [S1]. Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, with storage at +4°C or -20°C as instructed; components should not be used past the expiration date [S1].