Human Protein C ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the quantitative determination of human Protein C in plasma. The assay employs a pre-coated 96-well microplate (12 x 8 well strips), with standards and samples incubated alongside a biotinylated anti-Protein C antibody and an HRP-avidin conjugate. TMB substrate produces a colour change measured at 450 nm on a colorimetric microplate reader, with Protein C concentration calculated against the standard curve.

Across spiked sample panels, the kit reports average recovery of 91% in serum (range 80%–102%), 90% in EDTA plasma (range 81%–100%), and 84% in heparin plasma (range 80%–89%), with dilution linearity validated from 1:2 through 1:16. The assay delivers a sensitivity of less than 0.54 ng/ml across a working range of 1.25–80 ng/ml, with a total run time of approximately 4 hours 30 minutes.

Kit contents include the pre-coated microplate, Lyophilized Standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers. Components should be stored at +4°C or –20°C as indicated on each item, and the kit should not be used past its expiration date.