Human Protection Of Telomeres 1 Homolog ELISA Kit

This sandwich ELISA kit is designed for the in vitro quantitative measurement of human POT1 (Protection of Telomeres 1 Homolog) in tissue homogenates, cell lysates and other biological fluids. Distributed by Antibodies.com, the assay uses a pre-coated microplate and a biotinylated detection antibody followed by an HRP-avidin conjugate, with a TMB substrate read at 450 nm on a colorimetric microplate reader.

According to the manufacturer, the kit covers a quantitative range of 0.312–20 ng/ml with a sensitivity of <0.114 ng/ml and a total run time of 4 hours 30 minutes. Reported average recovery values across dilution series are 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5). Kit components should be stored at +4°C or –20°C as instructed and not used past the expiration date. Reactivity is confirmed for human samples.