Human Proteasome Subunit Alpha Type 5 ELISA Kit

Supplied by Antibodies.com under the Assays and Kits category, this immunoassay is configured as a Sandwich ELISA for the quantitative measurement of human Proteasome 20S alpha 5/PSMA5. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and is intended for use with a colorimetric microplate reader reading at 450 nm.

Samples including tissue homogenates, cell lysates and other biological fluids are added to the coated wells, and bound analyte is detected using a biotinylated antibody followed by an HRP-avidin conjugate and TMB substrate, producing a yellow product whose absorbance is proportional to target level. Reported total assay time is 4 hours 30 minutes. Capture and detection reagents are stored at -20°C, while wash buffer, TMB substrate and stop solution are stored at +4°C as instructed. The standard curve covers 0.312-20 ng/ml with a sensitivity of <0.114 ng/ml.

Validation data for serum (n=5), EDTA plasma (n=5) and heparin plasma (n=5) are provided, with average recovery ranges of 80%-102% (91%), 81%-100% (90%) and 80%-89% (84%) respectively across dilutions from 1:2 to 1:16. The product is intended for research use only and is not for therapeutic or diagnostic purposes.