Human Prostaglandin Endoperoxide Synthase 2 ELISA Kit

Sandwich ELISA kit from Antibodies.com for the in vitro quantitative determination of human COX2/Cyclooxygenase 2 in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated microplate arranged as 12 x 8 well strips, employing a biotinylated detection antibody, HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Reported standard range is 0.312–20 ng/ml with a sensitivity of <0.128 ng/ml, and the total assay time is 4 h 30 min. Sample recovery values stated by the manufacturer are: Serum (n=5) range 80%–102%, average 91%; EDTA plasma (n=5) range 81%–100%, average 90%; Heparin plasma (n=5) range 80%–89%, average 84%. Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4 °C or −20 °C as specified per component.