Human Proopiomelanocortin ELISA Kit

From Antibodies.com's Assays and Kits range, this Sandwich ELISA is listed for human reactivity with a target designation of Proopiomelanocortin. Associated manufacturer documentation describes the kit as a sandwich Enzyme-Linked Immunosorbent Assay for the in vitro quantitative determination of human ACTH in serum, plasma or other biological fluids.

The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips. An antibody specific for the target is immobilised on the plate; standards and test samples are added, followed by washing and incubation with a biotinylated anti-target antibody. An HRP-avidin conjugate is then added, and after further incubation and washing, TMB substrate is used to visualise the reaction, producing a blue product that turns yellow upon addition of acidic stop solution. The concentration is calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Quoted manufacturer figures include an assay range of 0.156-10 ng/ml and a sensitivity of <0.041 ng/ml, with a total assay time of 4h 30m. Reported sample recovery values are 91% average for serum (n=5, range 80% - 102%), 90% average for EDTA plasma (n=5, range 81% - 100%) and 84% average for heparin plasma (n=5, range 80% - 89%). Dilution linearity values include, for example, serum at 1:2 (n=5) ranging 87-91%, EDTA plasma at 1:2 (n=5) ranging 90-105% and heparin plasma at 1:2 (n=5) ranging 84-95%. Components listed include a pre-coated microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage at +4°C or -20°C as instructed; the kit should not be used past its expiration date.