Human Prolylcarboxypeptidase ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Prolylcarboxypeptidase (PRCP) in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated microplate, a biotinylated anti-PRCP detection antibody, an HRP-avidin conjugate, and a TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Reported performance figures include a sensitivity of less than 0.054 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery validation across sample matrices produced ranges of 80–102% in serum (average 91%), 81–100% in EDTA plasma (average 90%), and 80–89% in heparin plasma (average 84%), alongside dilution linearity assessed at 1:2, 1:4, 1:8, and 1:16. Kit components should be stored at +4°C or –20°C as indicated, and the assay must not be used past the stated expiration date.