Human Prolyl-4-Hydroxylase Alpha Polypeptide III ELISA Kit

Antibodies.com offers this sandwich ELISA kit for the in vitro quantitative determination of human P4HA3 (Prolyl 4 Hydroxylase Alpha Polypeptide III) in tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, together with a biotinylated anti-P4HA3 detection antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The reported assay sensitivity is 0.066 ng/ml and the standard curve covers 0.156-10 ng/ml, with a total protocol time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as indicated, and the kit should not be used past its expiration date.

Reported spike-and-recovery averages (n=5) are 91% for serum, 90% for EDTA plasma and 84% for heparin plasma, with corresponding ranges of 80-102%, 81-100% and 80-89%. Dilution linearity is reported across 1:2 to 1:16 for serum, EDTA plasma and heparin plasma samples.