Human Procollagen II N-Terminal Propeptide ELISA Kit

From Antibodies.com, this Human Procollagen II N-Terminal Propeptide ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human Procollagen II N-Terminal Propeptide in serum, plasma, tissue homogenates, or other biological fluids.

The assay uses a pre-coated microplate arranged as 12 x 8 well strips. Standards and samples are added to the wells, where the target is captured by an immobilized antibody. Following incubation and washing, a Biotinylated Anti-Procollagen II N-Terminal Propeptide Antibody binds the captured analyte, and HRP-Avidin conjugate is then added. TMB substrate solution is used to visualize the reaction, producing a blue colour that changes to yellow upon addition of acidic stop solution. The density of yellow is proportional to the amount of target captured, and concentration is calculated by reading the absorbance at 450 nm in a microplate reader against the standard curve.

Key specifications include a product range of 1.56–100 ng/ml, a sensitivity of 0.62 ng/ml, and an assay time of 4h 30m. Spike-and-recovery performance for serum ranged from 80% to 102% (average 91%), EDTA plasma 81% to 100% (average 90%), and heparin plasma 80% to 89% (average 84%) based on n=5 samples. Kit components include pre-coated 96 well microplate strips, lyophilized standard, Detection Solutions A and B, Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.