Human Procollagen II C-Terminal Propeptide ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human Procollagen II C-Terminal Propeptide in serum, plasma or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, using an immobilized capture antibody, a biotinylated detection antibody, and an HRP-avidin conjugate. Signal is generated with TMB substrate and quantified by measuring absorbance at 450 nm in a colorimetric microplate reader.

The kit is reactive with human samples, and components are stored at +4°C or -20°C. The reported assay range is 31.2-2000 pg/ml with a sensitivity of <13.6 pg/ml. Total assay time is 4h 30m.

Validation data include spike-and-recovery results for serum, EDTA plasma, and heparin plasma, along with dilution linearity for each sample type.