Human Procollagen I N-Terminal Propeptide ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Procollagen I N-Terminal Propeptide in serum, plasma and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and is intended for use with a colorimetric microplate reader, with absorbance measured at 450 nm.

Quantitative measurement is achieved via the sandwich enzyme immunoassay technique. Standards and samples are added to wells pre-coated with an antibody specific for Procollagen I N-Terminal Propeptide; captured analyte is then bound by a biotinylated anti-Procollagen I N-Terminal Propeptide antibody and detected using an HRP-avidin conjugate. TMB substrate solution produces a blue-coloured product that turns yellow after addition of the acidic stop solution, with the intensity of the yellow colour proportional to the amount of analyte present in each well.

The kit reports an assay range of 15.6-1000 pg/ml, a sensitivity of <6.4 pg/ml, and a total assay time of 4 hours 30 minutes. Kit components include a pre-coated 96-well microplate (12 x 8 well strips), 2 vials of lyophilized standard, 120 μl of Detection Solution A, 120 μl of Detection Solution B, 20 ml of 30X Wash Buffer, 45 ml of Sample Dilution Buffer, 9 ml of TMB Substrate, 6 ml of Stop Solution, and 5 adhesive plate sealers. Components are stored at +4°C or -20°C as instructed.