Human Pregnancy Zone Protein ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Pregnancy Zone Protein (PZP) in serum, plasma, tissue homogenates, or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and employs the sandwich enzyme immunoassay technique in which a PZP-specific capture antibody immobilises the target before sequential incubation with a Biotinylated Anti-PZP detection antibody and an HRP-Avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a yellow product whose density is proportional to PZP concentration and is read at 450 nm on a colorimetric microplate reader.

Quantitative performance characteristics quoted by the manufacturer include a sensitivity of 32.7 pg/ml and an assay range of 62.5–4000 pg/ml, with a total assay time of 4h 30m. Spike-and-recovery data from the manufacturer indicate average recoveries of 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (range 81–100%, n=5), and 84% in heparin plasma (range 80–89%, n=5), with dilution linearity (1:2 to 1:16) reported as 87–91% to 92–97% for serum, 90–105% to 79–108% for EDTA plasma, and 84–95% to 89–91% for heparin plasma. Kit components include a pre-coated microplate, lyophilised standard, Detection Solutions A and B, a 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage at +4°C or −20°C as instructed. The product is intended for research use only.