Human Potassium Chloride Cotransporters 4 ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human KCC4 (Potassium Chloride Cotransporters 4) in tissue homogenates and other biological fluids. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, a biotinylated anti-KCC4 detection antibody, and an HRP-avidin conjugate. Signal is generated with a TMB substrate, producing a blue product that turns yellow after addition of the acidic stop solution, and the absorbance is read at 450 nm in a colorimetric microplate reader.

Reported sensitivity is less than 0.061 ng/ml, with an assay range of 0.156–10 ng/ml and a total assay time of 4 hours 30 minutes. The kit is suitable for use with serum, EDTA plasma, heparin plasma, and tissue homogenates. Recovery data (n=5) are reported as 80–102% for serum (average 91%), 81–100% for EDTA plasma (average 90%), and 80–89% for heparin plasma (average 84%), with dilution linearity documented across 1:2 to 1:16 sample dilutions. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.