Human Poliovirus Receptor Related Protein 1 ELISA Kit

Designed for the in vitro quantitative determination of human PVRL1/NECTIN1 (Poliovirus Receptor Related Protein 1) in tissue homogenates, cell lysates or other biological fluids, this sandwich ELISA from Antibodies.com is reactive with human samples. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and is read colorimetrically at 450 nm on a standard microplate reader, with a total assay time of approximately 4 hours 30 minutes.

Reported performance characteristics include a sensitivity of <0.063 ng/ml and an assay range of 0.156–10 ng/ml. Recovery values from the manufacturer's documentation are listed for serum (n=5, 80%–102%, average 91%), EDTA plasma (n=5, 81%–100%, average 90%) and heparin plasma (n=5, 80%–89%, average 84%), along with dilutional linearity data at 1:2, 1:4, 1:8 and 1:16 dilutions for the same three sample types.

Supplied components comprise a pre-coated 96-well microplate, lyophilized standard (2 vials), Detection Solution A (120 µL), Detection Solution B (120 µL), 30X Wash Buffer (20 mL), Sample Dilution Buffer (45 mL), TMB Substrate (9 mL), Stop Solution (6 mL) and adhesive plate sealers, with individual component storage temperatures specified at +4°C or –20°C.