Human Podocin ELISA Kit

An ELISA kit supplied by Antibodies.com and listed under the Assays and Kits category is described by its manufacturer as a sandwich enzyme immunoassay for the in vitro quantitative determination of human NPHS2 in urine, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The Antibodies.com listing gives the target as Podocin and indicates reactivity with human samples. The pre-coated 96-well microplate platform (12 x 8 well strips) uses colorimetric detection read at 450 nm in a microplate reader, with a stated assay time of 4 hours 30 minutes.

Manufacturer specifications report a product range of 0.781–50 ng/ml and a sensitivity of <0.24 ng/ml. Reported recovery values are: serum (n=5) range 80–102%, average 91%; EDTA plasma (n=5) range 81–100%, average 90%; heparin plasma (n=5) range 80–89%, average 84%. Linearity-of-dilution ranges across 1:2, 1:4, 1:8 and 1:16 dilutions are also reported for the three sample types. Kit components include lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, stored at +4°C or -20°C as instructed.